Pourite Anti-Bubble Additive for Agar and Gel Media
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Traditional pH 4, 7 & 10, color coded.
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Pourite is an anti-bubble additive which prevents bubble formation in agar, or other
gel media such as electrophoresis gels.
Pourite, specially compounded from silicone and other resin emulsions, was developed
for its anti-foam properties, biocompatibility and inertness. Only 2-5 drops of
Pourite are required per liter of media to prevent bubble formation while pouring
plates and tubes or casing gel slabs. Greater speed is possible with automatic machines
when using Pourite. When Pourite is used, plates are not reopened for “flaming”
drastically reducing contamination from foreign organisms. This allows technicians
to focus on maintaining an aseptic environment.
Pourite is easy to use. Pourite is added to the water prior to adding the dry media.
The dry media is then added after Pourite has been well mixed with the water. Never
add to hot solutions. Media preparation is completed as usual followed by autoclaving
and the addition of any other nutrients. Pourite is inert and may be used with all
media. No refrigeration is necessary, and stability is over 2 years.
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This photograph illustrates a plate where Pourite was used along side an extreme
case of bubble entrapment which is not intended to represent typical experience
in anyone’s lab. However, Pourite has been doing a good for over twenty five years
in labs around the world.
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