Pourite is an anti-bubble additive
which prevents bubble formation in agar, or
other gel media such as electrophoresis gels.
Pourite, specially compounded
from silicone and other resin emulsions, was
developed for its anti-foam properties,
biocompatibility and inertness. Only 2-5
drops of Pourite are required per liter of
media to prevent bubble formation while
pouring plates and tubes or casing gel slabs.
Greater speed is possible with automatic
machines when using Pourite. When Pourite
is used, plates are not reopened for
“flaming” drastically reducing contamination
from foreign organisms. This allows
technicians to focus on maintaining an
aseptic environment.
Pourite is easy to use. Pourite is
added to the water prior to adding the dry
media. The dry media is then added after
Pourite has been well mixed with the water.
Never add to hot solutions. Media preparation
is completed as usual followed by autoclaving
and the addition of any other nutrients.
Pourite is inert and may be used with
all media. No refrigeration is necessary, and
stability is over 2 years.
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The photograph above illustrates a plate where
Pourite was used along side an extreme case of bubble
entrapment which is not intended to represent typical
experience in anyone’s lab. However, Pourite has been
doing a good for over twenty five years in labs around
the world.
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